Response of Leishmania chagasi promastigotes to oxidant stress
نویسندگان
چکیده
منابع مشابه
Reduced hamster usage and stress in propagating Leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation.
Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, beca...
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The immune responses of mice immunized with ovalbumin (OVA) together with killed L. major (KLM) promastigotes as adjuvant were studied. Three doses (5 × 107, 1 × 108 and 2 × 108) of KLM combined with OVA (100 mg) were injected into the groups of C57BL/6 mice. BCG and complete Freund’s adjuvant (CFA) were used as control adjuvants. Lymphocyte proliferation and antibody titers were determined, an...
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Previous works demonstrated that various species of Leishmania promastigotes exhibit differential sensitivity to complement-mediated lysis (CML) during development. Upon exposure to normal human serum (NHS), cultures of Leishmania chagasi promastigotes recently isolated from infected hamsters (fewer than 5 in vitro passages) are CML-sensitive when in the logarithmic growth phase but become CML-...
متن کاملLeishmania chagasi: homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model.
Homogenous metacyclic promastigotes of Leishmania chagasi were isolated by buoyant density from in vitro heterogeneous cultures and used for biochemical characterization of isoforms of the major surface protease (MSP). Compared to stationary phase promastigotes, metacyclic cells had three times more MSP, produced 3-fold higher parasite loads in a mouse model in vivo, and were more resistant to ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1994
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.62.11.5133-5141.1994